Good morning!
This week we dive into why we need high-throughput sequencing approaches and we will apply HT sequencing approaches to answer research questions. You will submit your first draft for your Group Project JoVE manuscript today, Monday, April 19 by 12noon. Throughout this week you will be giving peer feedback to other groups’ first draft of their Group Projects and submit that by Friday, April 23 by 5pm. As always, reach out if you have any questions or concerns including requests to meet with your Motivation Coach! And don’t hesitate to request a little grace to finish Module 6!
Best,
Your HTD Instructional Team
Overview
Why do we need HT approaches? In this module, we discuss considerations for high-throughput sequencing libraries that enable us to generate genomic sequences without prior knowledge of its sequence data. We will focus on the PCR based technologies that allow us to apply various approaches, their limitations and validation. The case study for this module asks us to help Dr. S solve an 80-year old mystery and will help us understand why we truly need HT approaches to genetic sequencing. The learning objectives for this module include identifying relevant literature and resources that can aid in the experimental design of assays using high-throughput sequencing approaches.
Upon successful completion of this module, you will be able to:
- Identify relevant literature and resources that can aid in an experimental design featuring high-throughput approaches. (CO 1, 2, 6)
- Describe several of the pros and cons of using high-throughput sequencing approaches as part of an experimental design. (CO 4)
- Explain how high-throughput technologies are necessary to generate data and findings to promote discovery. (CO 5)
To complete this module, you will need to:
- Fill in these chart notes while watching these three videos on:
- Traditional PCR (video opens in a new window)
- Sanger Sequencing (video opens in a new window)
- Illumina Sequencing (video opens in a new window)
- Complete the case study by answering questions on TopHat
