Good morning! In the next two Modules, we will dive into high-throughput sequencing and some of its applications. We will discuss an article that will help you prepare for a case study next week! We are also making significant progress on our Group Lab Projects, and we will continue to generate our JoVE-style manuscripts as we progress through Module 5!
Lab Project Updates
Last week you had a full lab session to collaborate with your research teams to complete Task 5 of the Group Lab Project, and this week we are working to complete Task 6. Teams are encouraged to frequently review the Group Lab Project Instructions and Rubric document to ensure that you are meeting timelines and content expectations! Below is a table of all the project tasks and due dates. You can read about these in greater detail in the Group Lab Project Instructions and Rubric!
| Task # | Task Descriptions | Suggested Deadlines |
|---|---|---|
| 1 | Complete Lab 1: Compound Screening and Growth Curves | Tuesday, March 7, from 12:50 – 5:50 pm |
| 2 | Complete Lab 2: Digital PCR | Tuesday, March 21, from 12:50 – 5:50 pm |
| 3 | Complete Lab 3: Sequencing | Tuesday, March 28, from 12:50 – 5:50 pm |
| 4 | Rank Lab Topic Interest & Assemble Research Teams | Friday, March 31, by 5 PM |
| 5 | Lab 4: JoVE Project | Tuesday, April 4, by midnight |
| 6 | Lab 5: JoVE Project: Submit a draft manuscript for peer review | Tuesday, April 11, by midnight |
| 7 | Submit peer reviews | Friday, April 14, by midnight |
| 8 | Lab 6: JoVE Project Final Day to video record | Tuesday, April 18, by midnight |
| 9 | Submit JoVE-ready files and public summary | Friday, April 28, by midnight |
Module 5 Overview
What are cutting-edge HT technologies? We are going to start addressing this question by reviewing high-throughput or next-generation sequencing (“NGS) and its many applications. We will then review a single-cell genomics application and search for other uses of high-throughput sequencing, paying particular attention to the throughput and technologies used as well as the advantages and limitations.
Upon successful completion of this module, you will be able to:
- Describe applications of HT sequencing technologies. (CO 1)
- Explain the methodology of a novel HT screening procedure and its utility in discovery. (CO 5)
- Identify and evaluate a cutting-edge screen. (CO 1, 4, 6)
To complete this module, you will need to:
- Watch Module 5 video (24 min).
- Respond to the Comprehension Check questions for Module 5.
- Participate in Forum 5 for JoVE 3 article.
- Complete the JoVE 3 Critical Thinking Scenario.
- Complete second milestone for the group project: first draft for peer review (Lab Task 6).
- Begin Peer Review for Milestone 3 deadline next week on 2/14.
- BIT 579: Submit a draft of NSF GRFP proposals for peer review.
Your HTD Instructional Team,
Dr. Carlos Goller | email: ccgoller@ncsu.edu
Dr. Carly Sjogren | email: casjogre@ncsu.edu
Dr. Phillip Brown | email: pebrown4@ncsu.edu
Blake Horton | email: tbhorton@ncsu.edu
