NC State

Week 6: Why we need high-throughput sequencing approaches

By Carlos C. Goller

Good afternoon! 

This week we dive into why we need high-throughput sequencing approaches and we will apply HT sequencing approaches to answer research questions. Today is the deadline to return your peer feedback to other Group Project drafts. Please submit that by 5 pm. As always, reach out if you have any questions or concerns including requests to meet with your Motivation Coach! And don’t hesitate to request a little grace to finish Module 6!

Best,

Your HTD Instructional Team

Dr. Carlos Goller | email: ccgoller@ncsu.edu 

Dr. Carly Sjogren | email: casjogre@ncsu.edu 

Illustration of yellow bulb jigsaw with two hands joining pieces. Collaboration from Flaticon.
Illustration of yellow bulb jigsaw with two hands joining pieces.

Collaboration from Flaticon.com

Overview

Why do we need HT approaches? In this module, we discuss considerations for high-throughput sequencing libraries that enable us to generate genomic sequences without prior knowledge of its sequence data. We will focus on the PCR-based technologies that allow us to apply various approaches, their limitations and validation. The case study for this module asks us to help Dr. S solve an 80-year old mystery and will help us understand why we truly need HT approaches to genetic sequencing. The learning objectives for this module include identifying relevant literature and resources that can aid in the experimental design of assays using high-throughput sequencing approaches. 

Upon successful completion of this module, you will be able to:

  1. Identify relevant literature and resources that can aid in an experimental design featuring high-throughput approaches. (CO 1, 2, 6)
  2. Describe several of the pros and cons of using high-throughput sequencing approaches as part of an experimental design. (CO 4)
  3. Explain how high-throughput technologies are necessary to generate data and findings to promote discovery. (CO 5)

To complete this module, you will need to: 

  • Fill in these chart notes while watching these three videos on:
  1. Traditional PCR (video opens in a new window)
  2. Sanger Sequencing (video opens in a new window)
  3. Illumina Sequencing (video opens in a new window)