Good morning! We hope you had a great weekend and are ready to learn more about high-throughput approaches. Last week we learned about high-throughput screening and introduced several definitions. This week we will focus on the question: what makes a good screen?
We estimate 3-4 hours of continuous work to complete this module. Remember: March 24 is a Wellness Day: No Classes! We want you to use that day to refresh, clear your mind, and come back with more ideas and examples to discuss with the group.
Overview
What makes a good screen? In this module, we will define key terms and differentiate target-based and phenotypic screens to evaluate when each is appropriate. We will discuss Z-factors and how they are used to determine the quality of a given assay.
Upon successful completion of this module, you will be able to:
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MO 2.1. Define the following terms: miniaturization and reproducibility. (CO 1)
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MO 2.2. Compare and contrast target-based vs. phenotypic screens, and determine situations in which each is appropriate. (CO 4)
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MO 2.3. Define and calculate Z-factor, and use it to determine the quality of a given assay. (CO 2)
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MO 2.3. Define and calculate Z-factor, and use it to determine the quality of a given assay. (CO 2)
To complete this module, you will need to:
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Watch the Module 2 video and review slides.
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Complete Case Study 1
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Respond to TopHat questions for Module 2
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Participate in Forum 2
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Enjoy the sunshine or relax on the wellness day
